The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. The text is supported by practical examples and carefully constructed chapter-ending exercises drawn from the authors' years of teaching experience, including easily adaptable Java code and completely worked out examples. Found insideNanostructured solar cells are very important in renewable energy sector as well as in environmental aspects, because it is environment friendly. This volume covers broad aspects of cell expansion in three different cell types: root hairs, pollen tubes, and hypocothyl cells. Found inside – Page 2035.0, Olympus, Tokyo, Japan) using identical settings. Fluorescence intensity of phalloidin-positive cells was quantified by using ImageJ (ver. This is a guide to imaging techniques for sedimentologists, paleolimnologists, paleoceanographers and microscopists involved in paleoenvironmental reconstruction. This the second volume of six from the Annual Conference of the Society for Experimental Mechanics, 2010, brings together 40 chapters on Microelectromechanical Systems and Nanotechnology. Found insideThe book will help readers discover the various facilities of ImageJ through a tutorial-based approach.This book is targeted at scientists, engineers, technicians, and managers, and anyone who wishes to master ImageJ for image viewing, ... Found inside – Page 6725 – ImageJ histograms of X - ray CT abnormal brain scans . ( a ) Histogram ... The Integrated Density represents the sum of the values of the pixels in the selection , being equivalent to the product of Area and Mean gray value . Median ( 97 ) ... The measurement results are presented in calibrated units ( Table 1 ) . Table 1 The ... Found inside – Page 357Images were acquired by computer by using WinView software ( Princeton Instruments ) and analyzed with ImageJ ( National Institutes of Health , Bethesda , MD ) . Fluorescence was calculated in intensity units integrated over the pixels ... This volume explores open-source based image analysis techniques to provide a state-of-the-art collection of workflows covering current bioimage analysis problematics, including colocalization, particle counting, 3D structural analysis, ... . This new edition covers x-ray production and interactions, projection and tomographic imaging, image quality, radiobiology, radiation protection, nuclear medicine, ultrasound, and magnetic resonance – all of the important physics ... Found inside – Page 98value of the fluorescent bacteria and the background with ImageJ (National ... formula: integrated density—(area of the cell × mean background readings). Found insideThe book provides a good introduction for beginners before looking in greater detail at more advanced material in selected areas. The volume highlights the importance of technique in gathering quantitative information. This coverage will enable medical oncologists and students as well as basic researchers to capture the up-to-date entire picture of human telomeres and telomerase in cancer. Found inside – Page 6673To assess this increase , the integrated density of PLK4 signals at maternal centrioles was quantified using ImageJ and expressed in arbitrary units ( Fig . 6A , right ) . A 6.9 - fold increase of endogenous PLK4 protein was detected in cells with ... This volume of the acclaimed Methods in Cell Biology series provides specific examples of applications of confocal microscopy to cell biological problems. Found inside – Page 12The rats were intrathecally injected with either insulin (0.2 units) or saline ... First, the integrated optical density (IOD) of the positive staining and ... Found inside – Page 81(B) The density of the blots of gmLiGPAT and gsdmLiGPAT were measured with ImageJ software and expressed in arbitrary optical density units. This new volume of Methods in Cell Biology looks at methods for analyzing of biophysical methods in cell biology. Found inside – Page 1Cone Beam Computed Tomography in Endodontics is designed to inform readers about the appropriate use of CBCT in endodontics, and enhance their clinical practice with this exciting imaging modality. Found inside – Page 125ImageJ does calculate area and pixel value statistics of user-defined selections and mea-sure distances and angles. It can create density histograms and ... This book explores the experimental techniques used to monitor movement of calcium ions into/out of cells and between subcellular compartments, as well as analyse its effects on the intracellular targets that mediate its effects. This second edition details new emerging areas of zebrafish research focusing on genetics and genomics, techniques for developing and analyzing zebrafish disease models, and methods for neuroscience. Found inside – Page iiThis third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques ... This book provides necessary background to develop intuition about why interest point detectors and feature descriptors actually work, how they are designed, with observations about tuning the methods for achieving robustness and invariance ... Found inside – Page iiThis text is a companion volume to Transmission Electron Microscopy: A Textbook for Materials Science by Williams and Carter. Found inside – Page iiiEnergy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. The unique approach of this book gives researchers the benefits of a north-south comparison as well as the integration of paleoecological histories, current ecosystem dynamics and modeling of future changes. This volume explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionised our ability to analyse cells, cellular clusters and populations. This e-book discusses the above mentioned and related questions. The book also serves as an important depository for state-of-the-art technologies, methods, theoretical simulations and innovative ideas and hypotheses for future testing. This volume provides an overview of advanced fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems. This volume covers some of the most widely used protocols on nanocanonical amino acids, providing details and advice for users to get each method up and running for their chosen application. This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Found inside – Page 73Relative fluorescence units were calculated using the following formula: corrected total fluorescence = integrated density – (ROI area background). Found insideProgress in Optics Volume 45. Found insideSetting out the principles of stereology from a statistical viewpoint, this book focuses on both basic theory and practical implications. Found insideThis Open Access textbook provides students and researchers in the life sciences with essential practical information on how to quantitatively analyze data images. Found inside – Page 203Fluorescence intensity of phalloidin-positive cells was quantified by using ImageJ (ver. 1.47q, Bethesda, MD, USA), and the results expressed as ... Found inside – Page iiThis book describes how man-made litter, primarily plastic, has spread into the remotest parts of the oceans and covers all aspects of this pollution problem from the impacts on wildlife and human health to socio-economic and political ... This volume addresses that gap, gathering multidisciplinary knowledge and providing tools for understanding the neuroscience techniques that are essential to the field, and allowing the reader to design experiments in a variety of ... Found inside – Page 12176Determination of cell body diameter, body area, and neurite length were done with the NIH ImageJ program. ... Semiquantification of SERCA2b-integrated density revealed a significant increase from 1 .0 ± 0.04 arbitrary units (a.u.) in control ... Found inside – Page 38The individual bands were selected and the integrated density was measured. ... Taxonomic Classification units/ml (EU/ml)] in liver samples were calculated. Found insideThis development has made them a fascinating object of scientific study. This volume gives an overview of how roots have adapted to the soil environment and which roles they play in the soil ecosystem. One of the modules in the EUTEMPE-NET course is entitled, 'Anthropomorphic phantoms for optimization of dose and image quality in radiology', making this book invaluable for both medical physicists and biomedical engineers as well as unique ... While there are a number of books on the qualitative identification of chemical substances by TLC, the unique focus here is on quantitative analysis. Zymography, the practice of detecting enzyme activity on electrophoretic gels in environmental,... 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